Mahtabani A¹ (MSc.), Bayat M² * (PhD), Hosseini S.E¹ (PhD), Aminafshar M³ (PhD), Tavakoli H⁴ (PhD)

  ¹ Department of Food and Science Technology, Faculty of Agriculture and Natural Resource,

  Science and Research Branch, Islamic Azad University, Tehran, Iran

  ² Department of Medical and Veterinary Mycology, Faculty of Specialized Veterinary Sciences,

  Science and Research Branch, Islamic Azad University, Tehran, Iran

  ³ Department of Animal Science, Faculty of Agriculture and Natural Resource,

  Science and Research Branch, Islamic Azad University, Tehran, Iran

  ⁴ Department of Food Hygiene, Baqiyatallah University of Medical Sciences, Tehran, Iran

  Received: 15 Jun 2010, Accepted: 10 Apr 2011

  Abstract

  Introduction: Fungi have ability to attack and contaminate plants and animals' dead or alive organs therefore, possibility of contamination of agricultural products is high. Alfatoxins and Ochratoxins are mostly produced by Aspergillus species such as A.flavus, A.parasiticus, A.nomius, A.orhizeus, A.orhizea, A.ochraceus and penicillium puberculum. In this study, we aimed to assess the rate of fungal Aflatoxins and Ochratoxin in breakfast grains presented in supermarkets in Tehran.

  Methods: Eighteen samples were randomly selected. The samples were grinded and separately packed. Then, they were assessed during three stages of extraction, purification, and determination. The diagnosis was done using fluorescence detector: λme: 333, λxe: 460 and grain: 1000, Attn: 16. The rate was determined comparing the under curve level of each sample and the standards.

  Results: No Ochratoxin was found in the samples (rate= 0.0). Aflatoxin B1, B2 were found in some samples however, the rates were below the standards of Iran (5 ppb), Europe (2- 4 ppb) and the US. (2ppb). The Aflatoxin G1 and G2 rates were 0.0 in our samples.

  Conclusion: There was positive correlation between reduced amount of fungi generating Aflatoxin and controlling factors with reduced Aflatoxin rates. The control factors can be used to prevent grain contamination to Aflatoxin.

  Key words: Ochratoxin, Aflatoxin, HPLC, Breakfast grains, Tehran

  Hakim Research Journal 2011 14(1): 10- 15.

  * Corresponding Author: Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Tel: +98- 912- 5874738 , Fax: +98- 21- 22110193, E-mail: Dr_mansour_bayat@yahoo.com